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KMID : 0363420100240030092
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Journal of Korean Oriental Pediatrics
2010 Volume.24 No. 3 p.92 ~ p.105
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The Effects of Daecheongryong-tang on Transcription Factors and Adipogenic Genes during 3T3-L1 Differentiation
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Cho Sung-Woo
Kang Kyung-Hwa
Lee Seung-Yeon
Yu Sun-Ae
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Abstract
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Objectives:The purpose of this study is to investigate the effects of Daecheongryong-tang (DCRT) on the adipogenesis in 3T3-L1 preadipocytes.
Methods: 3T3-L1 preadipocytes were differentiated with adipogenic reagents by incubating for 2 days in the absence or presence of DCRT ranging 0.25 and 2%. The effect of DCRT on adipogenesis was examined by Oil red O staining, and the protein, RNA, and RT-PCR were measured.
Results: Our results showed that DCRT decreased the TG content by ORO staining. To elucidate the mechanism of the effects of DCRT on lowering TG content in 3T3-L1 adipocytes, we examined the DCRT modulate expressions of transcription factors to induce adipogenesis and adipogenic genes which is related to the regulation of accumulation of lipids. As a result, the expression of SREBP1, C/, C/, C/, and genes, which induce the adipose differentiation and adipose-specific aP2, adipsin, LPL, CD36, TGF- and adiponectin genes which regulates fat formations, were decreased. In addition, DCRT reduced the expression of iNOS and IL-6 in 3T3-L1 adipocytes, resulting in inflammation.
Conclusions : DCRT could regulate transcript factor related to induction of adipose differentiation, inhibit the accumulation of lipids and expression of the adipogenic genes.
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KEYWORD
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Daecheongryong-tang (DCRT), Adipocytes, Differentiation, Transcript factor, Adipogenic genes
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